The role of Rad55, Rad57, and MAT heterozygosity in homologous recombination. Amy Mozlin

ISBN: 9780549859055

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NOOKstudy eTextbook

210 pages


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The role of Rad55, Rad57, and MAT heterozygosity in homologous recombination.  by  Amy Mozlin

The role of Rad55, Rad57, and MAT heterozygosity in homologous recombination. by Amy Mozlin
| NOOKstudy eTextbook | PDF, EPUB, FB2, DjVu, AUDIO, mp3, RTF | 210 pages | ISBN: 9780549859055 | 5.51 Mb

DNA damage in a cell threatens cell viability and genome integrity. The failure to repair DNA damage or aberrant repair can result in cell death or large-scale chromosomal aberrations such as deletions, translocations, and chromosome fusions thatMoreDNA damage in a cell threatens cell viability and genome integrity.

The failure to repair DNA damage or aberrant repair can result in cell death or large-scale chromosomal aberrations such as deletions, translocations, and chromosome fusions that lead to genome instability and are the trademarks of cancer cells.

Homologous recombination (HR) is a DNA repair pathway used to fix DNA double-strand breaks (DSBs) and single-stranded gaps (SSGs) in the context of a replication fork. These lesions can arise spontaneously or be induced by genotoxic agents.-In the yeast Saccharomyces cerevisiae, Rad51 is a key protein in the HR process and is thought to load onto ssDNA at the break site and promote pairing with homologous duplex DNA for repair.

Rad55 and Rad57 are known as the Rad51 paralogs because they share 20-30% sequence similarity to Rad5l and are thought to help mediate Rad51 nucleoprotein filament formation. Our studies have found an increased requirement for Rad55 and Rad57 in the repair of SSGs occurring during replication in contrast to the repair of DSBs.

This heightened importance of Rad55 and Rad57 in SSG repair might be due to an increased need for them in mediation of Rad51 filament formation or might indicate that the Rad51 paralogs have an ancillary role in SSG repair distinct from the Rad51 filament.

These findings are important because most endogenous DNA damage probably occurs during replication and understanding the mechanisms behind its repair could be useful in developing effective cancer therapeutics.-Cell-type in Saccharomyces cerevisiae is determined by the MAT locus and is typically one of three cell types.

In haploid form, it can be either MATa or MATalpha and diploids express both mating-type alleles, a/alpha.

It has been recognized for some time now that cells that have the MATa/alpha genotype are more proficient at homologous recombination between homologs. Our studies have confirmed this effect in the G1 phase of the cell cycle when diploids can only repair off of a homologue and not a sister chromatid.

Preliminary findings indicate that the MATa/alpha genotype might increase pairing of homologs during G1 thus making them more accessible templates for repair. Understanding yeast cell-type control of DNA repair might be important in understanding how higher eukaryotes could regulate DNA repair in differentiated cell types.



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